Prostate Specific Antigen anticorps

N° du produit ABIN3026028

L’anticorps Souris Monoclonal anti-Prostate Specific Antigen a été validé pour IHC (p), IF et FACS. Il convient pour détecter Prostate Specific Antigen dans des échantillons de Humain.

Aperçu rapide pour Prostate Specific Antigen anticorps (ABIN3026028)

Antigène: Prostate Specific Antigen (PSA)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Prostate Specific Antigen est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: A67-B-E3-1A7

Détail du produit anti-Prostate Specific Antigen anticorps

Purification: Protein G affinity chromatography

Immunogène: PSA (p30) from human sperm plasma was used as the immunogen for the Prostate Specific Antigen antibody cocktail.

Isotype: IgG

Information d'application

Indications d'application: Optimal dilution of the Prostate Specific Antigen antibody cocktail should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Stockage

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Prostate Specific Antigen antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Prostate Specific Antigen

Antigène: Prostate Specific Antigen (PSA)

Autre désignation: Prostate Specific Antigen

Sujet: Recognizes a single protein of 33-34  kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.

Pathways: Système du Complément